c-Jun (Ser63) II, c-Jun (Ser73) BioAssay(TM) Phosphorylation Detection Kit

C-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements (Figure 1). The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2). Extracellular signals including growth factors, transforming oncoproteins, and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73 (3, 4). The c-Jun (Ser63) II and c-Jun (Ser73) Antibody Kit provides reagents and protocols for the rapid analysis of c-Jun phosphorylation at Ser63 and at Ser73. The kit includes two phospho-antibodies that recognize c-Jun only when phosphorylated at Ser63 or Ser73, a c-Jun antibody (phosphorylationstate independent), protein controls and protein markers for western blots, and our HRP Western Detection System.\n\nApplications: \nSuitable for use in Western Blotting, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern blotting: 1:1000 \nImmunocytochemistry: 1:1000 \n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5815-15

Size

1Kit

Applications

ICC, WB

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

Purity

Purified by protein A and peptide affinity chromatography.

References

1. Binetruy, B., Smeal, T., and Karin, M. (1991) Nature 351, 122

Additional Info

Detects phosphorylated Ser63 of c-Jun but does not appreciably crossreact with the corresponding phosphorylated form of JunD or JunB. Phospho-c-Jun (Ser73) antibody detects phosphorylated c-Jun at Ser73 or JunD at Ser100, yet will not react with up to 0.5ug of non-phosphorylated c-Jun or JunD.\n\nSpecies crossreactivity: human, mouse.