c-Met, Phosphorylated (Tyr1230, Tyr1234, Tyr1235) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c-Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c-Met contains three tyrosines (Tyr-xx-\nx-Tyr-Tyr motif) within the activation loop of the catalytic domain. This is also seen with the insulin receptor, insulin-like growth factor receptor (IGF1) receptor and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation. With c-Met ( and the related family member, RON) phosphorylation of tyrosines 1234 and 1235 has been shown to be important in receptor activation. Activation of the c-Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin and GAB1) and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, Erk1&2, JNK, PLC-g and STAT3).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2000 detects phospho-Met (Tyr1230/Tyr1234/Tyr1235) in A431 cells treated with EGF.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M3007-16A

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hov H, et al. Clin Cancer Res. 10(19): 6686-94, 2004. 2. Longati P, et al. Oncogene 9(1): 49-57, 1994. 3. Palka HL, Park M, Tonks NK. J Biol Chem 278(8): 5728-35, 2003. 4. Chiara F, Michieli P, Pugliese L, Comoglio PM, J Biol Chem 278(31): 29352-8, 2003

Additional Info

Recognizes phosphorylated c-Met (Tyr1230/Tyr1234/Tyr1235). Species Cross-reactivity: human and mouse tested. Species sequence homology: rat-100%