C5aR (C5a Anaphylatoxin Chemotactic Receptor, C5a-R, CD88, C5ar1, C5r1)

The rat anaphylatoxin C5a, a 77aa glycopolypeptide generated by proteolytic cleavage of C5, is one of the most potent inflammatory chemo attractants and interacts with two C5a receptors, C5aR and C5L2. Most of the C5a effects are mediated through C5aR. C5aR is known to be crucial in the initiation of acute inflammatory responses. C5a receptor is a seven transmembrane GTP-binding-protein-coupled receptor that belongs to the rhodopsin supergene family. The 45kD C5a receptor is expressed by leukocytes such as neutrophils, eosinophils, basophils, monocytes, dendritic cells and mast cells. C5aR can be expressed on both immune and non-immune cells. The latter, include vascular endothelial cells, cardiomyocytes and bronchial epithelial cells. Signaling of C5aR involves intracellular calcium mobilization and activation of among others protein kinase B (PKB) and protein phospholipase D (PLD) pathways. High C5aR expression levels have been associated with the pathogenesis of many inflammatory conditions, autoimmune and neurodegenerative diseases like rheumatoid arthritis, multiple sclerosis, atherosclerosis and inflammatory bowel disease. In vivo blockade of C5aR greatly reduces inflammatory injury. Using immunohistology there is no evidence for non-myeloid expression in healthy control rats. However, under inflammatory situations, the C5aR was found to be upregulated in various organs and tissues including the liver. Applications: Suitable for use in Flow Cytometry, Western Blot, ELISA, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Flow Cytometry: 2x10e5 cells were incubated with mAb at 3-5ug/ml, all incubations on ice Immunohistochemistry (Frozen): 7um frozen paraformaldehyde fixed sections were blocked with serum and incubated with mAb at 5ug/ml Western Blot: cell homogenate was separated on 10% SDS-PAGE and blotted on nitrocellulose. After blocking blot was incubated with mAb (1:500-1:1000) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.