Home  >  Products  >  Calpain 1, Small Subunit (Calcium-activated Neutral Protease, Calcium-activated Neutral Proteinase 1, CANP, CANP I, CANP1, Capa-1, Capa1, Capn1,mu-Calpain, muCANP, muCL, u-Calpain)
Calpain 1, Small Subunit (Calcium-activated Neutral Protease, Calcium-activated Neutral Proteinase 1, CANP, CANP I, CANP1, Capa-1, Capa1, Capn1,mu-Calpain, muCANP, muCL, u-Calpain)

Calpain 1, Small Subunit (Calcium-activated Neutral Protease, Calcium-activated Neutral Proteinase 1, CANP, CANP I, CANP1, Capa-1, Capa1, Capn1,mu-Calpain, muCANP, muCL, u-Calpain)

Cat no: C1035-27J

Supplier: United States Biological
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Calcium-dependent cysteine proteinases, or calpains, are widely distributed in mammalian cells. There are 2 distinct molecular forms, calpains 1 and 2, which differ in the quantity of calcium required.Both calpains 1 and 2 are heterodimeric; each is composed of 1 heavy (about 80 kD), CAPN1 and CAPN2, respectively, and 1 shared light (about 30 kD) subunit, CAPNS1. The heavy subunit has a catalytic function, and the light subunit is regulatory (1). Applications: Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry andImmunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Western Blot: 1:1000-1:5000 Immunohistochemistry: 1:100-1:250 Immunocytochemistry: 1:100-1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: C1035-27J
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Online Mendelian Inheritance in Man (OMIM), John hopkins University, Calpain, small subunit 1; APNS1 (114170)
Additional info: Recognizes human Calpain. Species Crossreactivity: Mouse and rat.

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