Home  >  Products  >  Calpain 3 (Calpain-3, Calcium-activated Neutral Proteinase 3, CANP 3, CANP3, CAPN3, Calpain L3, CANPL3, Calpain p94, LGMD2, LGMD2A, Muscle-specific Calcium-activated Neutral Protease 3, New Calpain 1, NCL1, nCL-1)
Calpain 3 (Calpain-3, Calcium-activated Neutral Proteinase 3, CANP 3, CANP3, CAPN3, Calpain L3, CANPL3, Calpain p94, LGMD2, LGMD2A, Muscle-specific Calcium-activated Neutral Protease 3, New Calpain 1, NCL1, nCL-1)

Calpain 3 (Calpain-3, Calcium-activated Neutral Proteinase 3, CANP 3, CANP3, CAPN3, Calpain L3, CANPL3, Calpain p94, LGMD2, LGMD2A, Muscle-specific Calcium-activated Neutral Protease 3, New Calpain 1, NCL1, nCL-1)

Cat no: C1035-27V

Supplier: United States Biological
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Calpain 3 is a muscle-specific calcium-activated neutral protease. Defects in the gene encoding for calpain 3 appear to be the origin of a form of limb-girdle muscular dytrophy. This antibody will help detect the presence or absence of calpain 3 in western blotting and maybe useful in further characterizing calpain 3 expression. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:25-1:50 Optimal dilutions to be determined by the researcher. Positive Control: Freshly isolated normal human skeletal muscle homogenate prepared in treatment buffer containing 0.125M tris-HCl buffer pH6.4, 10% glycerol, 4% SDS, 4M urea, 10% mercaptoethanol and 0.001% bromophenol blue (final pH of treat- ment buffer, 6.8). Bands at approx- imately 94kD and 30kD. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C1035-27V
Reactivities: Human, Canine, Rabbit
Hosts: Mouse
Applications: Western Blot
Size: 2.5ml
Form: Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 2.5ml dH2O.
P type: Mab
Isotype: IgG2b
Purity: Supernatant
Alternative names: Calpain 3, (p94), EC=3.4.22.54
References: 1.Anderson L V B, Davison K, Moss J A, et al..
Additional info: Recognizes human Calpain 3. Species Crossreactivity: Canine and rabbit.

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