Carbohydrate Sulfotransferase 1 (CHST1, Carbohydrate (keratan sulfate Gal-6) sulfotransferase 1, C6ST, Carbohydrate Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1, GST-1, Keratan sulfate Gal-6 sulfotransferase, KS6ST, KSGal6ST, K

The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6 O or 4O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). CHST4, also\nknown as high endothelial cells Nacetylglucosamine 6Osulfotransferase (HECGlcNAc6ST)\nor L-selectin ligand sulfotransferases (LSST), catalyzes the transfer of sulfate to position 6 of non reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L selectin\nligands (4). It has a catalytic preference for core 2-branched mucin-type O glycans, but also has activity toward core 3 type of O glycan 5). Human CHST4 shares 72% amino acid sequence identity with the mouse ortholog.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1.0ug/ml\nWestern Blot: 1.0ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C1103-01

Size

100ug

Applications

ELISA, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS. \n

P Type

Pab

Purity

Purified by immunoaffinity chromatography.\n

Isotype

IgG

References

1. deGraffenried, D. and Bertozzi, C.R. (2003) J. Biol. Chem. 278: 40282.\n2. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.\n3. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.\n4. Bistrup, A. et al. (1999) J. Cell Biol. 145:899.\n5. Uchimura, K. et al. (2002) J. Biol. Chem. 277: 3979.\n6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V,\nAcademic Press, Inc., New York, 964.\n7. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613.\n8. Wu, Z.L., et al. (2002) Faseb J. 16:539.

Additional Info

Recognizes human CHST1.