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Carbonyl Reductase 1 (CBR 1, CRN, CBR, hCBR1)

Cat no: C1146-04

Carbonyl Reductase 1 (CBR 1, CRN, CBR, hCBR1)

Carbonyl reductase is one of several monomeric, NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds. This enzyme is widely distributed in human tissues. Another carbonyl reductase gene, CRB3, lies close to this gene on chromosome 21q.\n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:16,000\nWestern Blot: 0.2-0.5ug/ml; ~30-35kD band seen in Human Liver and Kidney lysates [Calculated MW of 30.4kD according to NP_001748.1.\nImmunohistochemistry: 1-2ug/ml; In paraffin embedded Human Kidney shows cytoplasmic staining of epithelial cells of renal tubules.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C1146-04

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1. Gonzalez-Covarrubias V, Zhang J, Kalabus JL, Relling MV, Blanco JG.\nPharmacogenetics of human carbonyl reductase 1 (CBR1) in livers from black and white donors.\nDrug Metab Dispos. 2009 Feb;37(2):400-7.\nPMID: 19022938\nThe goat polyclonal antibody used in this paper was manufactured by us:\nChoi I, Kim JY, Lee EJ, Kim YY, Chung CS, Chang J, Choi NJ, Chung HJ, Lee KH. \nOntogeny of expression and localization of steroidogenic enzymes in the neonatal and prepubertal pig testes.\nJ Androl. 2009 Jan-Feb;30(1):57-74.\nPMID: 18772489\nThe goat polyclonal antibody used in this paper was manufactured by us:\nGavelov

Additional Info

Recognizes human Carbonyl Reductase 1.

Alternative Names

short chain dehydrogenase/reductase family 21C, member 1, SDR21C1, prostaglandin-E(2) 9-reductase, prostaglandin 9-ketoreductase, hCBR1, carbonyl reductase (NADPH) 1, carbonyl reductase (NADPH), CBR, carbonyl reductase 1, CBR1

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