Cartilage Oligomeric Matrix Protein (COMP)

Cartilage oligomeric matrix protein (COMP) is a non-collagenous glycoprotein and is a member of the thrombospondin family of extracellular proteins. COMP is a calcium-binding protein of high molecular weight (>500kD) present in the extracellular matrix of articular, nasal and tracheal cartilage. COMP is not only cartilage-derived but was found widely in other tissues, including synovium and tendon. Intact COMP is pentameric, with five identical subunits and the carboxy-terminal globular domain of native COMP binds to collagens I, II, and IX. It has been proposed that COMP molecules are important for maintaining the properties and integrity of collagen network. In addition COMP may have a storage and delivery function for hydrophobic cellsignaling molecules such as vitamin D. The significance of COMP for normal development and function of cartilage has been underscored by the discovery that mutations of the COMP gene result in pseudoachondro-plasia and some forms of multiple epiphyseal dysplasia. Most published studies have shown that serum levels of COMP provide important information about metabolic changes occurring in the cartilage matrix in joint disease. These studies describe that serum COMP level correlated with cartilage degradation and is a potential prognostic marker in inflammatory joint diseases such as osteoarthritis (OA) and rheumatoid arthritis (RA). Results have demonstrated an association of increasing serum COMP levels with progressive destruction of articular cartilage monitored radiographically. OA and RA are a common disease causing pain and disability in a significant proportion of the adult population and early diagnostics of these diseases is very important for future therapy. \n\nApplications:\nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stabillty:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C1395-01

Size

100ug

Applications

ELISA, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Vilim V, et al. Arch Biochem Biophys. 1997 May 1;341 (1):8-16. 2. Misumi K, et al. Am J Vet Res. 2002 Apr;63 (4):598-603. 3. Jordan JM, et al. Arthritis Rheum. 2003 Mar;48 (3):675-81. 4. Lindhorst E, et al. J Orthop Res. 2000 Mar;18 (2):269-80. 5. Vilim V, et al. Clin Chim Acta. 2003 Feb;328 (1-2):59-69. 6. Dragomir AD, et al. Osteoarthritis Cartilage. 2002 Sep;10 (9):687-91. 7. Clark AG, et al. Arthritis Rheum. 1999 Nov;42 (11):2356-64. 8. Vilim V, et al. Osteoarthritis Cartilage. 2001 Oct;9 (7):612-8. 9. Vilim V, et al. Osteoarthritis Cartilage. 2002 Sep;10 (9):707-13.

Additional Info

Recognizes human Cartilage Oligomeric Matrix Protein (COMP).