Casein Kinase II (CKII, CK2, phosvitin kinase, troponin T kinase, casein kinase G) is a ubiquitous serine/threonine protein kinase that is highly conserved and commonly found in eukaryotic cells (found in the cytoplasm and the nucleus). CKII is a heterotetramer that consists of two catalytic subunits (alpha, alpha-prime) and two regulatory beta subunits (1). CKII can utilize both GTP and ATP as the phosphate donor and phosphorylates threonine and serine residues. Therefore, CKII is a multifunctional protein kinase with broad range of substrates (e.g. c-Myc, c-Myb, c-Fos, c-Jun, p53, Rb, HPVE7) and it has been implicated in various cell functions/processes from cell transformation to mitosis. (2)
Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:500 -1:2000
Immunohistochemistry: 1:100
Immunocytochemistry: 1:100
Flow Cytometry: 1:20
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
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