Caspase 11 (Casp4, Caspase 4, Aoptosis-related cysteine peptidase, Casp11, ASP-4, Caspase-4, Caspl, Ich3, ich-3, Protease ICH-3)

Caspase-11, (also known as CASP-11, ICH-3, ICE-B) promotes IL-b processing by procaspase-1 (ICE) and therefore may also have a role in inflammatory response as evident by a 30 fold increase in CASP-11 activity by LPS. The mouse and rat CASP-11 (373aa) are most homologous to human CASP-4 (377aa) protein (ICErel-II, TX, ICH-2) and could possibly be the ortholog of human CASP-4. The pro-or zymogen form of CASP-11 is activated by Granzyme-B or cathepsin-B and the processed enzyme consists of two large p20 (20kD) and two small ~p10 (10kDa subunits. CASP-11 is highly expressed in lung and spleen, weaker in heart and very little in liver, kidney, testis and skeletal muscle but has not been detected in the brain.\n\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: detects two bands of ~38kD and ~43kD.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2088-56D

Size

100ug

Applications

FC, WB

Hosts

Rat

Reactivities

Mouse, Rat

Form

Supplied as a liquid. Protein G affinity purified antibody in PBS containing 0.02% sodium azide.\n

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. J. Hur, et al.; FEBS Lett. 507, 157 (2001) 2. S.J. Kang, et al.; Cell Death Differ. 9, 1115 (2002) \n3. R. Schauvliege, et al.; J. Biol. Chem. 277, 41624 (2002)\n

Additional Info

Recognizes mouse caspase-11 (epitope in the p20 fragment). Species Crossreactivity: Rat