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Caspase 3  (CPP-32, Apoptain, Yama, SCA-1)

Cat no: C2087-22C3

Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)

Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kD) proenzyme. Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176 thereby generating two subunits of 17kD and 12kD, respectively. Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis. Activation of Caspase 3 occurs in response to variety of apoptotic inducers including Fas mediated apoptosis. \n\nApplications:\nSuitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions: \nImmunoprecipitation (Native verified): 2ug/mg of protein lysate (Use Protein A)\nWestern Blot: 1ug/ml for 2 hours at RT \nImmunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT\nNote: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nJurkat cells, Tonsil and appendix\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2087-22C3

Size

500ul

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2087-22C3X.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Krajewska M, et al. Cancer Res 1997 Apr 15; 57(8):1605

Additional Info

Recognizes human Caspase 3 (CPP32).

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