Caspase-9, Pro (CASP-9, Apoptotic Protease Mch-6, Apoptotic Protease-activating Factor 3, APAF-3, ICE-like Apoptotic Protease 6, ICE-LAP6, CASP9, MCH6)

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:2000\nImmunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000\nImmunoprecipitation: 1:50-1:200\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nBrain, spleen, lymphoid tissue, cancer cell lines\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2088-13B

Size

50ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Siegel RM. 2006. Caspases at the crossroads of immune-cell life and death. Nature 6:308-317. 2. Lavrik IN, A Golks, and PH Krammer. 2005. Caspases: pharmacological manipulation of cell death. J Clin. Invest. 115:2665-2672

Additional Info

Recognizes the proform of human caspase-9 (~50kD), and the large (~35kD) and small (~15kD) subunits of active/cleaved Caspase-9. Intermediate caspase-9 cleavage forms may also be seen at ~21kD. Species Crossreactivity: canine, gerbil, mouse, rat.