Catalase, Human, Western Blot Control

Highly reactive and potentially dangerous reactive oxygen species (ROS) are normally produced within the cells, primarily from the mitochondrial respiratory chain where in excess electrons are donated to molecular oxygen (o2) to generate peroxide anion (O2-). Superoxide anion is reduced by the superoxide dismutase (SOD) to hydrogen peroxide (H2O2) and hydrogen peroxide is reduced to water (H2O) by catalase, located primarily in the peroxisomes, and by glutathione peroxidase (GPx), located in the mitochondria and cytosol. Hydrogen peroxide, in the presence of transitions metals, can be converted to the highly toxic hydroxyl radical (OH.) and all three of the ROS (O2-, H2O2, and OH.) can damage macromolecules (proteins, DNA etc). The GPxs are commonly considered the most important for ROS defense since they have broader substrate specificities and stronger affinity for H2O2 than catalases.\nCatalase (CATA, 527 aa, chromosome 11p13) is peroxisomal enzyme found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. It is homotetramer. Defects in CAT are the cause of acatalasia or acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.\n\nSpecificity:\nRecognizes human Catalase. Species Crossreactivity: mouse.\n\nApplications:\nSuitable for use in Western Blot. Not suitable for use in ELISA or other applications where native protein is required as this preparation is not biologically active. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 10ul/ lane for good visibility with antibody Cat # C2096-05A. \nOptimal dilutions to be determined by the researcher.\n\nNote:\nSDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the solution prior to heating and loading on gels. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2096-05B

Size

50ul

Applications

WB

Form

Supplied as a liquid in SDS-PAGE sample buffer (reduced).

Purity

Purified

References

1. Bell GI et al (1986) Nucl Acid Res. 14, 5561-5562; 2. Ogata M et al (1991) Hum Genet. 86, 331-340; 3. Quan F et al (1986) Nucl Acid. Res. 14, 5321-5335; 4. Wen J et al (1990) J. Mol. Biol. 211, 383-393