CD154 CD8a, Fusion Protein (Biotin)

Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells. Interaction of CD154 and CD40 is essential for isotype switching in B cells. Known genetic defects that alter this interaction lead to impaired immune system function (1). CD154 has been shown to be hyperexpressed by B and T cells in SLE patients (4). CD154 has been reported to be expressed on vascular endothelial cells, smooth muscle cells and macrophages indicating a possible role for the CD40-CD154 immunoregulatory signaling mechanism during inflammation and immunity in atherogenesis (6). \n\n\nMolecular Structure: \nA soluble fusion protein consisting of the extracellular domain (213aa) of human CD154 fused to the extracellular domain (167aa) of murine CD8 alpha.\n\nApplications: \nSuitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.\n

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C2548-84N8

Size

25ug

Applications

ELISA, FC, WB

Conjugates

Biotin

Form

Supplied as a liquid in 50mM Sodium Phosphate, pH 7.5, 100mM Potassium Chloride, 150mM sodium chloride, 5% Glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with Biotin.

Purity

Purified by affinity chromatography.

References

1. D. Gray, et al, Seminars in Immunol 6: 303-310. (1994).2. F. Pietravalle, et al, J Biol Chemistry 271: 5965-5967. (1996). 3. R.J. Noelle, Immunity 4: 415-419. (1996). 4. A. Desai-Mehta, et al, J Clin Invest 97: 2063-2073.(1996). 5. I.S. Grewal and R.A. Flavell, Immunol Today 17: 410-414. (1996). 6. F. Mach, et al, Proc Natl Acad Sci USA 94:1931-1936.(1997).