CD2 (E Rosette Receptor, T11, Lymphocyte Function Antigen 2, LFA-2, Sheep Erythrocyte Receptor)

CD2 which is also known as E-rosette receptor, T11 and lymphocyte-function antigen-2 (LFA-2) is expressed on T cells, thymocytes, and subset of natural killer cells. Human CD2 functions as the receptor for sheep erythrocytes, human CD58 (LFA-3), and CD15s (Sialyl Lewis X). p56lck, p59fyn, CD3eta and CD3epsilon are tyrosine-phosphorylated\nafter CD2 stimulation. CD2 plays a role in T cell activation, T- or NK-cell-mediated cytolysis,\napoptosis in activated peripheral T cells, and regulation of T cell anergy. Normal B lymphocytes, monocytes or granulocytes do not express surface CD2 antigen, neither do common ALL cells. The antigen has been characterized as the receptor for sheep erythrocytes and CD2 monoclonal antibodies that inhibit E rosette formation. \n\nAntigen distribution: T cells (E+) 100% Thymocytes 95 + 3% Peripheral blood lymphocytes 82 + 5% NK cells (CD16+) 50 + 10% Granulocytes < 1% Monocytes < 1% B Cells (E-) < 1% \n\nRecommended Secondary Reagents: Goat anti-mouse IgG: HRP conjugate (rat absorbed), IgG Goat anti mouse IgG: RPE (rat adsorbed)\n\nFusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSO myeloma cell line.\n\nRecommended Negative Controls: Mouse IgG1 Negative Control \n\nApplications:\nFlow Cytometry: 1:10-1:50\nImmunohistochemistry (frozen)\nOptimal working dilutions to be determined by researcher.\n\nFlow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.

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SPECIFICATIONS

Catalog Number

C2253-07E

Size

250ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes the rat CD2 cell surface antigen, expressed by thymocytes and peripheral T cells. * Note that for \"general purpose\" detection of rat CD2 by flow cytometry.