CD235ab (Glycophorin A/B, GpA, GpB)

CD235a (Glycophorin A (GpA)) antigen is exclusively expressed on human erythroid cells and their progenitors. These antibodies may be effectively used for differentiation in acute leukemias e.g. presence of the CD235a antigen on leukemia cells indicates an early erythroid cell lineage of the tumor cells. CD235a antibodies are used to identify erythroid cells during hematopoietic differentiation. CD235a is not expressed on lymphoid or granulocytic progenitor cells and therefore is very useful as a marker for detection of the erythroid cell lineage. CD235a seems to be absent from erythroid colony forming cells in bone marrow, but is present during maturation from pro-erythroid blast cells to mature erythrocytes. CD235a is clinically important in the classification of leukemias. Using Anti-CD235a antibodies, a distinction can be made between erythroleukemia and other (acute) leukemias like e.g. myeloid, lymphoid or undifferentiated leukemias. Anti-CD235a is often used in combination with detection of H-antigen and/or anti-CD36 antibodies for additional characterization of early erythroid cells. Additional immunophenotyping of early erythroleukemias can be performed by detecting myeloid-associated antigens such as CD13, CD14, CD15, CD33 and CD34, to discriminate from lymphoid lineage-associated antigens like CD2, CD7, CD10 and CD19.\n\nApplications:\nSuitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence: Indirect\nFlow Cytometry: 0.015ug/ 100ul peripheral blood\nImmunohistochemistry: Staining of acetone-fixed frozen sections and formalin

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C2548-98H

Size

100ug

Applications

FC, IF, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b,k

References

1. Shen DC., Tang MH., Zhang JX., et al., 1993. HIR2: A monoclonal antibody against glycophorin A. J. of Monoclonal Antibody. 1993: 9(2): 59. 2. David M., A. Pascale, B. Armand, et al., 2002. Leukocyte Typing VII: White Cell Differentiation Antigens. P577-582.\nGeneral references: 1. Andersson, L.C. et al. 1979. Glycophorin A as a cell surface marker of early erythroid differentiation in acute leukemia. Int.J.Cancer, 23, 717-720 2. Loken, M.R., et al., 1987. Flow cytometric analysis of human bone marrow: I. Normal erythroid development. Blood, 69, 255-263 3. Liszka, K. et al., 1983, Glycophorin A expression in malignant hematopoiesis. Am.J.Hematol., 15, 219-226 4. Villeval, J.L. et al., 1986, Phenotype of early erythroblastic leukemias. Blood, 68, 1167-1174 5. Cuneo, A., et al. 1990. Morphologic, immunologic and cytogentic studies in erythroleukemia: evidence for multilineage involvement and identification of two distinct cytogenetic-clinicopathological types. Br. J. Hematol., 75, 346-35.

Additional Info

Recognizes N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoprotein. Reacts with normal human RBC (95.9%). Does not react with peripheral blood lymphocytes, granulocytes and monocytes.