Home  >  Products  >  CD247 (CD247 Molecule, CD3H, CD3Q, CD3-ZETA, CD3Z, T-cell Surface Glycoprotein CD3 zeta Chain, TCRZ, T-cell Receptor T3 zeta Chain, T3Z) (FITC)
CD247 (CD247 Molecule, CD3H, CD3Q, CD3-ZETA, CD3Z, T-cell Surface Glycoprotein CD3 zeta Chain, TCRZ, T-cell Receptor T3 zeta Chain, T3Z) (FITC)

CD247 (CD247 Molecule, CD3H, CD3Q, CD3-ZETA, CD3Z, T-cell Surface Glycoprotein CD3 zeta Chain, TCRZ, T-cell Receptor T3 zeta Chain, T3Z) (FITC)

Cat no: C2548-99V1

Supplier: United States Biological
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The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non- covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits, all of which are required for efficient surface expression. T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events. A novel monoclonal antibody, named G3, has been developed that is specific for the TCR zeta chain subunit (CD247). The antibody is capable of precipitating both non-phosphorylated and tyrosine phosphorylated TCR zeta chain and may be used to detect TCR zeta expressing cells using Flow Cytometry on permeabilized cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels of a neat working dilution to 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Catalogue number: C2548-99V1
Reactivities: Mouse
Hosts: Mouse
Applications: Flow Cytometry
Size: 100ug
Form: Supplied as a liquid in PBS, 0.09% sodium azide, 1% BSA. Labeled with FITC.
P type: Mab
Isotype: IgG2a
Purity: Purified
References: 1. van Oers, N.S.C. et al. (1994) Production and characterisation of monoclonal antibodies specific for the murine T cell receptor zeta chain. J. Immunol. Methods 170: 261-268. 2. Anderson, P. (1995) mAb reative with lymphocyte restricted intracellular antigens in Leucocyte Typing V p325, Oxford University Press.
Additional info: Recognizes mouse zeta TCR subunit.

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