CD273 (Programmed Cell Death 1 Ligand 2, PD-1 Ligand 2, PD-L2, PDCD1 Ligand 2, Programmed Death Ligand 2, Butyrophilin B7-DC, B7-DC, PDCD1LG2, B7DC, PDCD1L2, PDL2)

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PDCD-1 that mediate inhibitory signals from the APC. PDCD-1 can bind two different but related ligands, PDL-1 and PDL-2, both of which are thought to act as a negative regulator of T cell activation. However, it has been suggested that PDL-2 can act to stimulate an immunogenic response through an alternative receptor from PDCD-1. At least three isoforms of PDL-2 are known to exist; this antibody is specific to the larger isoform. PDL-2 antibody has no cross-reactivity to PDL-2.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRaji cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2549-21K

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Holling TM, et al (2004) Hum. Immunol. 65:282-90. 2. Ishida Y, et al (1992) EMBO J. 11:3887-95. 3. LaGier J, et al (2006) Hum. Immunol. 67:568-78. 4. Zhang Y, et al (2006) Proc. Natl. Acad. Sci.\nUSA. 103:11695-700. 5. Liu X, et al (2003) J. Exp. Med. 197;1721-30.

Additional Info

Recognizes human PDL-2. Species Crossreactivity: mouse and rat.