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CD3 (Early T Cell Marker)

Cat no: C2254-13

CD3 (Early T Cell Marker)

The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intracytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells and is present in majority of T cell neoplasms. CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-All. \n\nApplications: \nSuitable for use in ELISA and to enumerate T cells in peripheral blood and tissue. Other applications not tested.\n\nRecommended Dilution: \nOptimal dilutions to be determined by the researcher.\n\nSource: \nAscites\n\nHybridoma: \nHybridization of X63.653 myeloma cells with spleen cells from BALB/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2254-13

Size

1mg

Applications

ELISA

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Puri?ed by ion exchange (DEAE) chromatography.

Isotype

IgG2b

Additional Info

Recognizes human CD3 antigen (MW 26/20/16kD).

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