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CD32/CD16 (Fc gamma Receptor II/III, FcRII/III) (PE)

Cat no: C2384-14

CD32/CD16 (Fc gamma Receptor II/III, FcRII/III) (PE)

Applications: \nSuitable for use in Flow Cytometry. Other applications not tested. \n\nRecommended Dilution: \nFlow Cytometry: Neat, 1:10 Use 10ul to label 10e6 cells in 100ul.\nOptimal dilution determined by the researcher\n\nHybridoma: \nCells from immunized rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.\n\nRecommended Negative Controls: \nRat IgG2b\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2384-14

Size

100Tests

Applications

FC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a lyphilized powder in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. \nConjugated to R. Phycoerythrin (RPE).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

Additional Info

Recognizes an epitope expressed by the murine low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32). In the mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes.

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