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CD32 (Fc gamma Receptor II, FcRII, Monocyte Activation Antigen gp40) RIIb, phosphorylated (Tyr292)

Cat no: C2384-02F

CD32 (Fc gamma Receptor II, FcRII, Monocyte Activation Antigen gp40) RIIb, phosphorylated (Tyr292)

Low affinity IgG Fc receptor gamma II (Fc-gamma II), also known as CD32, is a member of the immunoreceptor tyrosine-based activating/inhibitory motif (ITAM/ITIM) family. Fc gamma receptors play a critical role in immunity by linking the IgG antibody mediated response with the regulatory functions of the immune system (1). Three classes of Fc gamma receptor exist; Fc gamma RI (CD64, a high affinity receptor), Fc gamma RII, and RIII (CD32 and CD16 respectively, both low affinity receptors). Fc gamma RIIa is an activating receptor, while RIIb1, RIIb2, and RIIb3 are inhibitory receptors generated by alternative splicing (1-2). Fc gamma RIIa is critical for antigen-presenting-cell activation and Fc gamma RIIb downregulates B-cell functions (3). Fc gamma RIIb has also been known to block the inflammatory immune response in mice (4). Once phosphorylated on tyrosine residues, Fc gamma RIIb associates with SHIP to downregulate phagocytosis.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:50,000-100,000 \nImmunoprecipitation: 1:70 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

C2384-02F

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Ravetch J, et al. Annu Rev Immunol. 19: 275-90, 2001. 2. Tsubata T. (1999). Curr Opin Immunol 11, 249

Additional Info

Recognizes Fc gamma RIIb phosphorylated on tyrosine 292.

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