CD38 (Acute Lymphoblastic Leukemia Cells Antigen, ADP Ribosyl Cyclase, CADPr Hydrolase 1, Cyclic ADP Ribose Hydrolase, Ecto Nicotinamide Adenine Dinucleotide Glycohydrolase, I19 (Mouse), Lymphocyte Differentiation Antigen)

The CD38 antigen is also known as T10 antigen. The CD38 antigen is a 45kD single-chain type II glycoprotein., It is an integral membrane protein with a long extracellular C-terminal domain, a single membrane-spanning region and a short N-terminal cytoplasmic tail. The CD38 antigen is expressed on a variety of hematopoietic cells. Its distribution depends on the state of the cell differentiation and the cell activation. In adults, the CD38 molecule is expressed on earlier stage of B lymphocyte ontogeny, lost during maturation and re-expressed upon terminal differentiation to plasma cells. This molecule is also strongly expressed on thymocytes. It is found at low density on resting T lymphocytes. It is expressed on the majority of resting NK cells and monocytes. It is also found on platelets and red blood cells.\n\nApplications: \nSuitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:100. Use 10ul to label 10e6 cells in 100ul.\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nNS1 myeloma cells with spleen cells from Balb/c mice.\n\nRecommended Negative Controls: \nMouse IgG1 Negative Control \n\nRecommended Secondary Reagents: \nF(ab')2 rabbit anti-mouse IgG HRP\nF(ab')2 rabbit anti mouse IgG: RPE conjugate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50% ) glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2390-01B

Size

200ug

Applications

FC, IHC, IP

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in Tris buffered saline, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Barclay, A.N., et al.: The Leucoctye Antigen Facts Book. Academic Press. London (1997). 2. Berthelier, V., et al., Research in Immunology147(6): 407-11 (1996). 3. Chen, Z., Shen, D.C., Yu, A.X., et al.: Shanghai J. of Immunol. 7(1): 1 (1987). 4. Ellis, J.H., et al. J. Immunol. 155: 925-937 (1995). 5. Harada, N.L., et al. J. Immunol. 151: 3111-3118 (1993). 6. Howard, M., et al. Science 262: 1056-1059 (1993). 7. Kirkham, P.A., et al. Immunology 83: 513-516 (1994). 8. Kishimoto, T., et al., eds.: Leucocyte Typing Workshop VI. Garland Publishing, Inc. (1998). 9. Knapp, W., et al., eds.: Leucocyte Typing Workshop IV. Oxford University Press (1989). 10. Ling, N.R., et al. Eds.: Oxford Univ. Press 302-335 (1987). 11. Lund, F., et al.: Immunol. Today 16: 469-473 (1995). 12. Malavasi, F., et al. Immunol. Today 15: 95-97 (1994). 13. McMichael, A.J., Beverly, P.C.L., Gilks, W., et al., eds.: Leukocyte Typing III: White Cell Differentiation Antigens. pp41, 57, 66. Oxford University Press, New York (1987). 14. Mehta, K., et al.FASEB J. 18: 1408-1417 (1996). 15. Mikaelian, I., et al. Toxicology Pathology 32: 181-191 (2004). 16. Oliver, A.M., et al. J. Immunol. 158: 1108-1115 (1997). 17. Ramaschi, Blood 6(87): 2308-2313 (1996). 18. Santos-Argumedo, L., et al. J. Immunol. 151: 3119-3130 (1993). 19. Schlossman, S., Bloumsell, L., Gilks, W., et al., eds.: Leukocyte Typing V: White Cell Differentiation Antigens. pp249, 269. Oxford University Press, New York (1995). 20. Shen, D.C., Chen, Z., Yu, A.X., et al.: Chinese J. of Immunology 2(6): 331 (1986). 21. Yamada, M., et al., Brain Research 756(1-2): 52-60 (1997). 22. Yang, C.Y., Shen, D.C., She, M., et al.: J. Monoclonal Antibody 9(4): 56 (1993). 23. Zocchi, E., et al. Biochem. Biophys. Res. Com. 3(196): 1459-1465 (1993).

Additional Info

Recognizes human CD38 a 45kD glycoprotein and has a reactivity pattern consistent with antibodies of the CD38 cluster. CD38 is expressed by plasma cells, monocytes, early lymphoid cells and activated T cells.