Monoclonal antibodies clustered as CD3 recognize the constant structure of the CD3/TcR (T cell receptor) complex. This complex is present on mature T cells and during thymopoiesis. CD3/TcR is called the T cell receptor complex. This complex is composed of different structures, i.e. TcRa, TcRb, TcRg, TcRd, CD3g, CD3d, CD3e, the x-chain, and the h-chain. Less than 10% of human peripheral T cells express the g/d TcR complex. The function of a/b and g/d heterodimers is the recognition of peptide antigen bound to MHC, followed by T cell activation. Recent data suggest that CD3 chains are able to function autonomously in signal transduction. T cells play a major role in activation processes of the immune system. Detection of these cells is important during immune monitoring. In addition, a large number of leukemias are T cell-related disorders, in which antibodies recognizing CD3/TcR complex are important diagnostic tools. Differentiation between AML, T-ALL and B-ALL can be made by immunophenotyping with (early) differentiation markers. Cell surface detection using CD3 antibodies can be applied for detection of mature T-ALL, while immature T-ALL express cytoplasmic (cyCD3) but no surface CD3. CD3 antibodies are used, in characterization of various subtypes of chronic lymphoid leukemias. Examples of these chronic T cell leukemias are T-CLL (Sezary Syndrome) and the peripheral T cell lymphoma (ATLL) which co-express CD2, CD3, CD4 and CD5 antigens.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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