CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4

Recommended Secondary Reagents: F(ab')2 rabbit anti-rat IgG HRP, F(ab')2 rabbit anti-rat IgG: RPE conjugate\n\nHybridoma: Spleen cells from immunized DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.\n\nApplications:\nFlow Cytometry: 1:50-1:100\nImmunohistochemistry (frozen)\nOptimal working dilutions to be determined by researcher.\n\nFlow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood.\n\nStorage and Stability: May be stored at 4e showing negligible reactivity with E+ cells, monocytes, granulocytes, EBV-transformed B cell lines, and mouse splenocytes.\n\nCD4-positive T-cells provide helper funciton in pokeweed mitogen driven B-cell differentiation. Moreover, anti-CD4 selectively blocks the autologus mixed lymphocyte reaction (AMLR) with minimal or no effect on the allogeneic MLR (allo-MLR). Blocking of the autoreactivity occurs when either autologus B-lymphocytes or macrophages are used as stimulators. Specificity closely approximates the binding spectrum of OKT4 and Leu-3 antibodies both of which also recognize 55 to 65% of human peripheral blood T-cells.\n\nRecommended Secondary Reagents: F(ab')2 rabbit anti-rat IgG HRP, F(ab')2 rabbit anti-rat IgG: RPE conjugate\n\nHybridoma: Spleen cells from immunized DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.\n\nApplications:\nFlow Cytometry: 1:50-1:100\nImmunohistochemistry (frozen)\nOptimal working dilutions to be determined by researcher.\n\nFlow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood.\n\nStorage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2255-27R

Size

250ug

Applications

FC, IHC

Hosts

Rat

Reactivities

Can

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Cobbold, S. & Metcalfe, S. (1994). Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens 43(3): 137-154.2. Gorman, S.,

Additional Info

Recognizes the canine CD4 cell surface antigen. The antibody has been shown to block MHCII dependent T cell responses in vivo and in vitro. In the canine CD4 is expressed by neutrophils as well as by lymphocytes subsets.