Home  >  Products  >  CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein)
CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein)

CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein)

Cat no: C2399-07C

Supplier: United States Biological
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The CD45 cell surface antigen is also known as the leukocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10-1:100. 10ul labels 10e6 cells in 100ul. Routinely tested on human peripheral blood leukocytes. Immunohistochemistry (Frozen): 1:200 Immunohistochemistry (Paraffin): 1:200. Requires antigen retrieval using heat treatment (sodium citrate buffer, pH 6) prior to staining. Western Blot: Detects bands of ~180kD, 200kD, 210kD and 220kD in B-cell lysates. Optimal dilutions to be determined by the researcher. IHC Positive Control Tissue: Human Tonsil Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2399-07C
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Western Blot
Size: 200ug
Form: Supplied as a liquid in PBS, 0.09% sodium azide.
P type: Mab
Isotype: IgG2b
Purity: Purified by Protein G affinity chromatography.
References: 1. Alsinet, E. et al. (1990) Differential effects of anti-CD45 monoclonal antibody on human B cell proliferation: a monoclonal antibody recognizing a neuraminidase-sensitive epitope of the T200 molecule enhances anti-immunoglobulin-induced proliferation. Eur. J. Immunol. 20: 2801-2804. 2. del Conde, I. et al. (2005) Tissue-factor-bearing microvesicles arise from lipid rafts and fuse with activated platelets to initiate coagulation. Blood. 106: 1604-1611
Additional info: Recognizes all forms of human CD45 expressed by all hematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes.

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