Home  >  Products  >  CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein)
CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein)

CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein)

Cat no: C2399-07G2

Supplier: United States Biological
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CD45 or Leukocyte Common Antigen (LCA/T200) family is comprised of at least four isoforms (CD45RA, CD45RB, CD45RC, and CD45RO) of membrane glycoproteins with MW of 180-220kD. It consists of a large, heavily glycosylated, amino-terminal extracellular domain of 400-550 amino acids, a single transmembrane region and a large carboxy-terminal cytoplasmic domain of 700 amino acids. CD45/LCA is expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portion of CD45 proteins has phosphatase activity and is involved in regulation of transmembrane signals, functioning as a leukocyte membrane receptor capable of transducing signals via its phosphotyrosine phosphatase domains. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul Immunohistochemistry (Frozen): 1:500-1:1000 Immunohistochemistry (Paraffin): 1:100-1:200. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA, pH8.0 is recommmeded. Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Recommended Positive Control: Human Tonsil Recommended Negative Control: I1904-83P1: IgG2a Murine Negative Control Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2399-07G2
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
References: 1. Dalchau, R. et al. (1980) Eur. J. Immunol. 10: 737-744. 2. Quenby, S et al. (1999) Human Reprod. 14(9):2386-2391. 4. Hauser, P.V. et al. (2010) Am J Pathol. 177: 2011-21. 5. Mallam, E. et al. (2010) Mult Scler. 16: 909-18.6. Marrinucci, D. et al. (2010) J Oncol. 2010: 861341.7. Kazane, S.A. et al. (2012) Proc Natl Acad Sci U S A. 109: 3731-6. 8.. Carrade, D.D. et al. (2012) Cell Medicine. 4: 1-11.
Additional info: Recognizes human CD45. Reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. Species crossreactivity: equine

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