Home  >  Products  >  CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein)
CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein)

CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein)

Cat no: C2399-07N

Supplier: United States Biological
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CD45 (LCA) common leukocyte antigens are members of the T200 glycoprotein complex. CD45 antigen is a protein tyrosine phosphatase. CD45 antigen is present on all human leukocytes and progenitors in bone marrow. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen): 1:10 Immunohistochemistry (paraffin): 1:10. Does not require antigen retrieval. Immunohistochemistry (resin): 1:10 Requires antigen retrieval using heat treatment . Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C2399-07N
Reactivities: Porcine
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 1mg
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Purified by Ion Exchange chromatography.
References: 1. Binns, R.M. et al. (1995) Genetically determined CD45 variant of value in leucocyte tracing in vivo in the pig. Immunology 86: 25-33. 2. Goodchild, T. et al. (2006) Safety of intramyocardial injection of autologous bone marrow cells to treat myocardial ischemia in pigs. Cardiovasc Revasc Med. 7: 136-45. 3. Anttila, A. et al. (2003) T-cell-mediated mucosal immunity is attenuated in experimental necrotizing enterocolitis. Pediatr Surg Int. 19: 326-30. 5. Richter, Y. et al. (2004) Dynamic flow alterations dictate leukocyte adhesion and response to endovascular interventions. J Clin Invest. 113: 1607-14. 6. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update. Vet Res. 39: 54.
Additional info: Recognizes an allotypic determinant of porcine CD45. Reacts with Large White and German Landrace porcine.

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