CD45, also known as the leukocytes common antigen (LCA) consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180-240kD. Antibodies recognizing a common epitope on all of these isoforms are termed CD45, whilst those recognizing only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all hematopoietic cells, except erythrocytes. CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. This product is routinely tested by flow cytometry on rat splenocytes.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.