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CD45RO (PE)

Cat no: C2400-69

CD45RO (PE)

The CD45RO antigen is present on most thymocytes, about 40% of resting peripheral blood T-lymphocytes and the majority of T-cells in skin reactive in?ltrates and T-cell malignancies. CD45RO is also found on a subset of B-cells and some B-cell lymphomas. NK cells do not express the CD45RO antigen. It is also present on monocytes, macrophages and granulocytes. Low MW (Mr 180kD) isoform of the leukocyte common antigen (LCA) is a part of a family of protein tyrosine phosphatases. When activated with phytohemagglutinin (PHA), naive T-cells lose the CD45RA antigen and begin to express the CD45RO antigen. CD45RO may be a marker for memory T-cells.\n\nApplications: \nSuitable for use in Immunohistochemistry and Flow Cytometry. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunohistochemistry: Paraffin and frozen sections \nFlow Cytometry: 1ug to stain ~1x10e6 cells\nOptimal dilutions to be determined by the researcher.\n\nLabel:\nR-phycoerythrin has an absorbance maximum of 565.5nm. This ?uorochrome has been covalently conjugated and puri?ed chromatographically to remove unconjugated antibody and dye, while achieving a ?uorochrome/protein (F/P) molar ratio between 0.7-1.3.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Stable for 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

C2400-69

Size

200ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with R-phycoerythrin (PE).

P Type

Mab

Purity

Purified by affinity chromatography.

Isotype

IgG2a

References

1. Terry, L.A. et al (1988) Immunology 64:331 2. Smith, S.H. et al (1986) Immunology 58:63. 3. Norton, A.J. et al (1986) J. Clin. Pathol. 39:399.

Additional Info

Recognizes the low MW isoform of the leukocyte common antigen (LCA) (Mr 180kD) which is a part of a family of protein tyrosine phosphatases.

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