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$CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha IV, Integrin alpha 4, IA4, ITGA4, LPAM23, MGC90518, Very Late Activation Protein 4 Receptor Alpha 4 Subunit, VLA4, VLA-4)\n$

Supplier:

United States Biological

Cat no: C2404-71B

CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha IV, Integrin alpha 4, IA4, ITGA4, LPAM23, MGC90518, Very Late Activation Protein 4 Receptor Alpha 4 Subunit, VLA4, VLA-4)\n

CD49d is expressed on most lymphocytes, granulocytes, monocytes and thymocytes. The primary ligands for CD49d are CD106 (VCAM-1), fibronectin and MAdCAM-1. Reported to significantly inhibit binding of CD49d to CD106 but only when used in combination with clone R1-2 (1). Removal of sodium azide is recommended prior to use in functional assays. \n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry (frozen). Other applications not tested. \n\nRecommended Dilution: \nFlow Cytometry: 1:10-1:50, Use 10ul to label 10e6 cells in 100ul.\nOptimal dilution determined by the researcher. \n\nHybridoma: \nSpleen cells from immunized Lewis rats were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.\n\nRecommended Secondary Reagents: \nF(ab')2 Goat anti rat IgG:FITC (mouse adsorbed) \nF(ab')2 Goat anti rat IgG:RPE (mouse adsorbed) \n\nImmunohistochemistry: \nRecommended Secondary Reagents: \nGoat anti rat IgG:HRP (mouse adsorbed) .\n\nRecommended Negative Controls: \nRat IgG2a\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2404-71B

Size

250ug

Applications

FC, IHC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.\n

Isotype

IgG2a

References

1. Kinashi, T. et al. (1994). Adhesion Molecules in Hematopoietic Cells. Blood cells. 20:25-44

Additional Info

Recognizes murine alpha 4 integrin (CD49d), a 150kD glycoprotein that can associate with either beta 1 integrin (CD29) or beta 7 integrin to form heterodimers CD49d/CD29 (VLA-4) and alpha4/beta7 (LPAM-1) respectively.