Home  >  Products  >  CD53 (Antigen MOX44 Identified by Monoclonal Antibody MRC OX44, Cell Surface Glycoprotein CD53, Leukocyte Surface Antigen CD53, MOX44, Tetraspanin 25, Tetraspanin-25, TSPAN 25, TSPAN25, TSPAN-25)
CD53 (Antigen MOX44 Identified by Monoclonal Antibody MRC OX44, Cell Surface Glycoprotein CD53, Leukocyte Surface Antigen CD53, MOX44, Tetraspanin 25, Tetraspanin-25, TSPAN 25, TSPAN25, TSPAN-25)

CD53 (Antigen MOX44 Identified by Monoclonal Antibody MRC OX44, Cell Surface Glycoprotein CD53, Leukocyte Surface Antigen CD53, MOX44, Tetraspanin 25, Tetraspanin-25, TSPAN 25, TSPAN25, TSPAN-25)

Cat no: C2408-11E

Supplier: United States Biological
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Applications: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ul Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher. Hybridoma: NSO myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2408-11E
Reactivities: Rat
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
Alternative names: OTTHUMP00000059505, Transmembrane Glycoprotein
References: 1. Paterson, D.J. et al. (1987) The MRC OX-44 antigen marks a functionally relevant subset among rat thymocytes. J. Exp. Med. 165: 1-13. 2. Patterson, D.J. and Williams, A.F. (1987) An intermediate cell in thymocyte differentiation that express CD8, but not CD4 antigen. J. Exp. Med. 166: 1603-1608. 3. Tomlinson, M.G. et al. (1993) Epitope mapping of anit-rat CD53 monoclonal antibodies. Implications for the membrane orientation of the transmembrane 4 superfamily. Eur. J. Immunol. 23: 136-140.
Additional info: Recognizes rat CD53 cell surface antigen, a 43kD glycoprotein, which is a member of the transmembrane 4 super family. It is expressed by all myeloid and peripheral lymphoid cells, and by a small subset of thymocytes.

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