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CD53

Cat no: C2408-11

CD53

Anti-CD53 is expressed by all myeloid and peripheral lymphoid cells, and by a small subset of thymocytes.\n\nRecommended Secondary Reagents: Goat anti-mouse IgG: HRP conjugate (rat absorbed), IgG Goat anti mouse IgG: RPE (rat adsorbed)\n\nFusion Partners: Spleen cells from an immunized BALB/c mouse were fused with cells of the mouse NSO myeloma cell line.\n\nRecommended Negative Controls: Mouse IgG1 Negative Control \n\nFlow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. \nImmunohistochemistry: Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.\n\nApplications:\nFlow Cytometry: 1:100-1:200\nImmunohistochemistry: Frozen, Paraffin.\nImmunoprecipitation

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SPECIFICATIONS

Catalog Number

C2408-11

Size

250ug

Applications

FC, IHC, IP

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Paterson, D.J. et al. (1987). The MRC OX-44 antigen marks a functionally relevant subset among rat thymocytes. J. Exp. Med. 165: 1-13.\n\n\n\n2. Patterson, D.J. and Williams, A.F. (1987). An intermediate cell in thymocyte differe

Additional Info

Recognizes the rat CD53 cell surface antigen, a 43kD glycoprotein, which is a member of the transmembrane 4 super family.\n\nClone OX-44 recognizes a conformational epitope that is dependent on disulphide bonding (3).

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