CD54 (Intercellular Adhesion Molecule, ICAM-1) (BSA & Azide Free)

ICAM-1 permits antigen-independent adhesion between lymphocytes and their targets and may be used to study cell-cell interactionsor for rhinovirus and malarial studies. The LFA-1 binding site is located in domain 1 of ICAM-1. CD54 antigen is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macrophages and granulocytes. ICAM-1 has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells and on a variety of other cell types including activated fibroblasts, EC, macrophages and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. Inhibits adhesion of infected RBCs and LFA-1 to ICAM-1. CD54 is the receptor for rhinoviruses and malaria.\n\nApplications:\nSuitable for use in Blocking Assays, Flow Cytometry, and Immunohistochemistry (Acetone-fixed frozen sections). Other applications not tested.\n\nRecommended Dilution:\nBlocking Assays: Blocks binding of LFA-1 and infected RBCs to ICAM\nFlow Cytometry (direct): Use10ul of conjugated Ab/10e6 cells.\nFlow Cytometry (indirect): Use 0.5ug unconjugated Ab/10e6 cells.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nTonsil\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2409-20X

Size

100ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and sodium azide. See C2409-20.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Bailly, P., et al., PNAS USA 91: 5306-5310 (1994). 2. Fujita, H., et al., Archives of Biochemistry & Biophysics 309: 62-69 (1994). 3. Berendt, A.R., et al., Cell 68(1): 71-81 (1992). 4. J. Cell. Biol. 116: 1527-1535 (1992).

Additional Info

Recognizes human CD54/ICAM-1 at 85-115kD, depending on cell type.