CD54 (Intercellular Adhesion Molecule, ICAM-1) (PE)

ICAM-1 permits antigen-independent adhesion between lymphocytes and their targets and may be used to study cell-cell interactionsor for rhinovirus and malarial studies. The LFA-1 binding site is located in domain 1 of ICAM-1. CD54 antigen is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 antigen is strongly expressed on B-cells, T-cells, macophages and granulocytes.\n\nICAM-1 has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells and on a variety of other cell types including activated fibroblasts, EC, macrophages and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. Inhibits adhesion of infected RBCs and LFA-1 to ICAM-1. CD54 is the receptor for rhinoviruses and malaria.\n\nApplications: \nSuitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1ug to stain 1x10e6 cells\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.

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SPECIFICATIONS

Catalog Number

C2409-28

Size

100ug

Applications

FC, IHC, IP

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Labeled with PE.

P Type

Mab

Purity

Purified by Protein G affinity chromatography

Isotype

IgG2a

References

1. Kuhlman, P. et al. (1991) J. of Immunol. 146(6):1773. 2. Schynius, A. et al. (1993) J. Immunol. 150:655 3. Harlan, J. M. and Liu, D. Y. (1992) Adhesion: Its Role in In?ammatory Disease, W. H. Freeman and Co., New York, p. 154

Additional Info

Recognizes the Mr 95kD cell surface glycoprotein known as mouse intracellular adhesion molecule ICAM-1. ICAM-1 is the receptor for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18).