CD57 (BSA & Azide Free)

CD57/HNK-1 is expressed on a subpopulation of 15-20% of peripheral blood mononuclear cells, ~60% of NK active cells and on a subset of T cells.\n\nApplications: \nSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilution:\nImmunohistochemistry (Frozen & formalin/paraffin): 2-4ug/ml for 30 min at RT. Note: No special pretreatment is rquired for staining of formalin-fixed, paraffin embedded tissues.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nLymph node or tonsil\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2411-26X

Size

100ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C2411-26.

P Type

Mab

Purity

Purified by ammonium sulfate precipitation.

Isotype

IgM,k

References

1. Loy TS; Darkow GV; Spollen LE; Diaz-Arias AA. Immunostaining for Leu-7 in the diagnosis of thyroid carcinoma. Archives of Pathology and Laboratory Medicine, 1994, 118:172-4.\n2. Burger D; Steck AJ; Bernard CC; Kerlero de Rosbo N. Human myelin/oligodendrocyte glycoprotein: a new member of the L2/HNK-1 family. Journal of Neurochemistry, 1993, 61(5):1822-7.\n3. Guarino M. Plexiform schwannoma. Immunohistochemistry of Schwann cell markers, intermediate filaments and extracellular matrix components. Pathology, Research and Practice, 1993, 189(8):913-20.\n4. Liu XH; Yoshiki T; Kokuho M; Okada Y; Tomoyoshi T; Higuchi K. The prognostic value of the HNK-1 (Leu-7) antigen in prostatic cancer

Additional Info

Recognizes CD57 at 110kD. Species Crosseactivity: Human.