CD57 (CD57 Antigen, Beta-1,3-glucuronyltransferase 1, B3GAT1, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Glucuronosyltransferase P, GLCATP, GlcAT-P, GlcUATP, GlcUAT-P, HNK1, HNK-1, LEU7, LEU7 Antigen, NK1, NK-1, UDP-GlcUA Glycopr

CD57 (HNK-1) is an antigen associated with natural killer cells. It is also found in T-cell subsets and B cell subsets. This antibody is similar to HNK-1 and Leu-7. It detects CD 57 antigen (110kD) on the surface of NK cells in germinal centers, and almost 20% of peripheral blood mononuclear cells in adult. It may cross react to myelin-associated glycoprotein of peripheral nerves and prostate surface antigen. This antibody may be useful for the study of NK cell-mediated cytotoxity and functional properties of NK cells and T-cell subsets. \n\nApplications:\nSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilution:\nFlow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul\nImmunohistochemistry (frozen): 1:10-1:100\nImmunohistochemistry (paraffin): 1:10-1:50 Does not require antigen retrieval using heat treatment prior to staining sections but sodium citrate buffer may enhance staining.\nOptimal dilutions to be determined by the researcher. \n\nHybridoma: \nP3.X63 Ag8.653 myeloma cells with spleen cells from BALB/c mice.\n\nRecommended Secondary Antibody:\nI1905-01D: IgM (AP) Pab. Gtx Mo\n\nRecommended Negative Control:\nI1905-26C: IgM Murine Negative Control\n\nRecommended Positive Control (IHC):\nHuman Tonsil\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2411-23C

Size

100ug

Applications

FC, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgM

References

1. Funaro, A. et al. (1995) Epitope analysis of human CD57 by means of a panel of newly-generated high-affinity murine monoclonal antibodies. In Leucocyte Typing V: White Cell Differentiation Antigens, 2. Funaro, A. et al. (1995) Human CD57, a link molecule between leucocyte and neural cells. In: Leucocyte Typing V: White Cell Differentiation Antigens.

Additional Info

Recognizes the human CD57 molecule expressed by NK cells and a subset of T cells.