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CD57 (HNK-1, NK (Natural Killer ) Cells)

Cat no: C2411-32

CD57 (HNK-1, NK (Natural Killer ) Cells)

CD57 antigen is present on 15-20% of normal peripheral blood mononuclear cells. It is expressed on a subset of natural killer cells (60%) and on a subset of T-lymphocytes. This carbohydrate is also present on N-CAM in the nervous system.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: Formalin-fixed paraffin embedded tissue sections.\nFlow Cytometry: 1ug stains 1x10e6 cells.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nHybridization of P3-NSI/1-Ag4-1 myeloma cells with spleen cells from Balb/c mice\n\nSource: \nTissue culture\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2411-32

Size

200ug

Applications

FC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Fel

Form

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgM

References

1. Arimatsu, Y., et al., J. Neuroscience 7: 1250 (1987). 2. Barnstable, C., et al., Brain Research 559: 118 (1995). 3. Abo, T., Balch, C.M., et al., J. Immunol. 127: 1024 (1981).

Additional Info

Recognizes a (Mr 110kD) carbohydrate molecule on feline myeloid cells. This surface antigen is associated with myelin-associated glycoprotein (MAG). Blocks binding of HNK-1 (Leu.7) antibody. Species Crossreactivity: human.

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