CD58 (LFA-3)

CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes [1]. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells [2]. There are approximately 5000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria. \n\nRecommended Secondary Reagents:F(ab')2 rabbit anti mouse IgG: RPE conjugate\n\nRecommended Negative Controls: Mouse IgG2a Negative Control\n\nApplications:\nFlow Cytometry: 1:10\nWestern Blot \nOptimal working dilutions to be determined by researcher.\n\nFlow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.

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SPECIFICATIONS

Catalog Number

C2412-06A

Size

200ug

Applications

FC, WB

Hosts

Mouse

Reactivities

Hum

Form

Purified IgG - liquid with 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Makgoba, M.W. et al. (1989). Immunology Today 10: 417-422.\n\n\n\n2. Shaw, S., Johnson, J.P., (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens. Edited by Knapp, W., Dorken, B., Gilks, W.R., Rieber, E.P., Schmidt, R.

Additional Info

3H1578 was produced in response to erythrocytes. The functional affinity of 3H1578 binding to erythrocytes is 4 x 10e8 M-1. It reacts by immunoblotting to non-reduced erythrocyte membranes. 3H1578is an indirect haemagglutinin. The antigen on erythrocytes is Pronase sensitive. 3H1578 inhibits T cell rosetting.