CD59 (16.3A5, 1F5, CD59a, EJ16, EJ30, EL32, FLJ38134, FLJ92039, G344, 20kD Homologous Restriction Factor, HRF20, HRF-20, Ly-6-like Protein, Lymphocytic Antigen CD59/MEM43, MAC Inhibitory Protein, MAC-IP, MEM43 Antigen, Membrane Attack Complex (MAC) Inhibi

The antibody is the prototypic antibody for the CD59 determinant, a PI-linked glycoprotein of 18-20kD, which is broadly expressed on most types of of cells. The antigen is the major complement-protecting molecule of human cells. It is expressed on all leukocytes, erythrocytes and most other cell types. Binding of this antibody inhibits the complement-protecting activity of CD59. CD59 is one of the complement regulatory membrane proteins that is missing in Paroxysmal Nocturnal Haemoglobinuria (PNH). Anti CD59 reagents may therefore be useful in the differential diagnosis of PNH type II and PNH type I as reported. \n\nApplications: \nSuitable for use in Flow Cytometry, Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: Non-reducing conditions\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C2413-22E

Size

100ug

Applications

FC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Horejsi V. et al. 1986 Folia Biol., 34, 12. 2. Leucocyte Typing IV, ed. W. Knapp et al., Oxford Univ. Press, Oxford 1989; Workshop nr N706. See Stefanova I. et al. p. 678-680. 3. Leucocyte Typing V., Schlossman S. et al., 1995, Oxford University Press. 4. Forsberg U.H. et al., 1989, Immunogenetics, 30, 188. 5. Stefanov

Additional Info

Recognizes human CD59.

Alternative Names

1F5 Antigen, 20kD Homologous Restriction Factor, CD59 Antigen, CD59 Antigen p18 20, CD59 Glycoprotein, CD59 Molecule, CD59 Molecule Complement Regulatory Protein, Human Leukocyte Antigen MIC11,