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CD59 (MACIF, MIRL, Protectin) (Azide Free)

Cat no: C2413-21E

CD59 (MACIF, MIRL, Protectin) (Azide Free)

Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti mouse IgG: RPE conjugate\n\nRecommended Negative Controls: Mouse IgG2a Negative Control: Azide Free\n\nApplications:\nFlow Cytometry: 1:5-1:10\nWestern Blotting: Non-reducing\nImmunohistochemistry: frozen, paraffin.\nELISA (7)\nWestern Blot\nRadioimmunoassay \nOptimal working dilutions to be determined by researcher.\n\nFlow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. \nImmunohistochemistry: Recommended positive control tissue: Tonsil.

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SPECIFICATIONS

Catalog Number

C2413-21E

Size

1mg

Applications

ELISA, FC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Stefanova, I., et al. (1989) in Leucocyte Typing IV: White cell differentiation antigens. Ed. Knapp, W. et al. Oxford University Press pp 678-697.\n\n\n\n2. Stefanova, I. et al., (1989), Mol. Immunol. 26: 153-161.<

Additional Info

Reacts with a PI-linked glycoprotein, Mw 18-20kD found on all types of leucocytes including platelets. The antigen is expressed on many haemapoietic and non-haemapoietic cells. 3H1587 has been shown to stimulate NK activity. * The epitope recognized by 3H1587 is lost after reduction, therefore, non-reducing conditions are required for western blotting techniques (2, 5).

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