CD68 (Macrosialin, DKFZp686M18236, Macrophage Antigen, SCARD1, Scavenger Receptor Class D Member1)

Recognizes CD68 which is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. Reacts with myeloid precursors, peripheral blood granulocytes and plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. Shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained.\n\nApplications: \nSuitable for use in Immunohistochemistry and Immunofluorescence. Not recommened for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Paraffin): 1:40-1:80. Requires antigen retrieval using heat treatment prior to staining of paraffin sections; 1mM EDTA, pH 8.0, is recommended.\nImmunohistochemistry: Frozen sections\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \np3-NS1-Ag4-1 myeloma cells with spleen cells from Balb/c mice. \n\nPositive Control: \nHuman Tonsil \n\nRecommended Secondary Antibodies:\nI1904-65W: IgG, F(ab

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SPECIFICATIONS

Catalog Number

C2423-05

Size

1ml

Applications

IF, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.

P Type

Mab

Purity

Supernatant

Isotype

IgG2a

References

1. Hameed, A., et al., Human Pathology 25: 872-876 (1994). 2. Madden, L.R., et al., Proc. Natl. Acad. Sci. USA Aug 9, 2010 [Epub ahead of print]. 3. Muthana, M., et al., Cancer Res. Jan 13, 2011 [Epub ahead of print]. 4. da Costa, C.E., et al., J. Exp. Med. 201: 687-693 (2005). 5. Wang, X., et al., J. Transl. Med. 4: 30 (2006). 6. Coury, F., et al., Nat. Med. 14: 81-87 (2008). 7. Rodriguez-Agudo, D., et al., J. Lipid Res. 47: 1168-1175 (2006). 8. Achard, V., et al., Am. J. Physiol. Regul. Integr. Comp. Physiol. 292: R274-R282 (2007). 9. Paulmyer-Lacroix, O., et al., Eur. J. Endocrinol. 155: 177-185 (2006). 10. Hever, A., Proc. Natl. Acad. Sci. USA 104: 12,451-12,456 (2007). 11. Silaghi, A., et al., Am. J. Physiol. Endocrinol. Metab. 293: E1443-E1450 (2007). 12. Kaibara, N., et al., Histopathology 52: 856-864 (2008). 13. Moskovskzy, L., et al., Genes Chromosomes Cancer 48: 468-479 (2009). 14. Angel, C.E., et al., Int. Immunol. 19: 1271-1279 (2007).

Additional Info

Recognizes the human CD68 cell surface antigen, a 110kD glycoprotein primarily expressed by macrophages and monocytes.

Alternative Names

DKFZp686M18236, Macrophage Antigen CD68 (Microsialin), GP110, SCARD1, Scavenger Receptor Class D Member 1