CD68 (MACROSIALIN, DKFZp686M18236, Macrophage antigen, SCARD1, Scavenger receptor class D member1) (FITC)

Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation. Does detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested. \n\nRecommended Dilution: \nFlow Cytometry: Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required for this application.\nOptimal dilution determined by the researcher\n\nRecommended Negative Controls: \nMouse IgG1 Negative Control: FITC\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2423-05B

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide. Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC).

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Kreipe, H. et al. (1987). Ki-M7 Monoclonal Antibody Specific for Myelomonocytic Cell Lineage and Macrophages in Human. J. Histochem. Cytochem. 35(10): 1117-1126. 2. Diop, O.M. et al. (2000). High Levels of Viral Replication during Primary Simian Immunodeficiency Virus SIVagm Infection Are Rapidly and Strongly Controlled in African Green Monkeys. J. Virology 74(16): 7538-7547. 3. Micklem, K. et al. (1989). A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies. Brit. J. Haem 73: 6-11. 4. Glushakova, S.et al. (1999). Nef Enhances Human Immunodeficiency Virus Replication and Responsiveness to Interleukin-2 in Human Lymphoid Tissue Ex Vivo. J. Virology 73(5): 3968-3974. 5. Sadovnikova, E. et al. (2002). The CD68 protein as a potential target for leukaemia-reactive CTL. Leukemia 16(10): 2019-2026.

Additional Info

Recognizes the 110KD integral membrane glycoprotein CD68 (Macrosialin) predominantly expressed on the intracellular lysosomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukaemia studies.

Alternative Names

DKFZp686M18236, Macrophage antigen CD68 (microsialin), GP110, SCARD1, Scavenger receptor class D member1