CD90 (Thy-1.1)

Thy-1 was discovered in early 1960s during the search for heterologous antisera against mouse leukemia cells. It was originally named theta (0) antigen, then Thy-1 (THYmocyte differentiation antigen 1) due to its prior identification in thymocytes (precursors of T cells in the thymus). The human homolog was isolated in 1980 as a 25kDa protein (p25) of T-lymphoblastoid cell line MOLT-3 binding with anti-monkey-thymocyte antisera. The conserved gene and its alleles Thy-1 has been conserved throughout vertebrate evolution and even in some invertebrates, with homologs described in many species like squid, frogs, chickens, mice, rats, dogs, and humans. The Thy-1 gene is located at human chromosome 11q22.3 (mouse chromosome 9qA5.1). Some believe that there may be a functional significance of both this gene and CD3 delta subunit (T3D) mapping to chromosome 11q in man and chromosome 9 in mouse, though there is no homology (in fact this speculation lead to its localization in chromosome 11q-the human chromosome region syntenic to mouse chromosome 9 which harbored T3D). In mouse there are two alleles Thy1.1 (Thy 1a, CD90.1) and Thy1.2 (Thy 1b, CD90.2) which differ by one amino acid at position 108 (arginine in Thy-1.1 and glutamine in Thy-1.2). Thy 1.2 is expressed by most strains of mice, whereas Thy1.1 is expressed by some like AKR/J and PL mouse strains.\n\nApplications:\nSuitable for use in Immunocytochemistry as a cytotoxic and staining antibody. Extremely efficient for killing in vivo. Other applications not tested.\n\nRecommended Dilution:\nImmunocytochemistry: For staining, use 10ul/10e6 cells. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2441-54A

Size

500ug

Applications

ICC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 0.5ml of dH2O or 40-50% glycerol..

P Type

Mab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgG1

References

1. Skeletal Muscle Pump vs. Respiratory Muscle Pump: Modulation of Venous Return from the Locomotor Limb in Humans. Miller JD, Pegelow DF, Dempsey JA, J Physiol 2005 Jan 13; 2. Evidence for enhanced bioavailability of trace elements in the marine ecosystem of Deception Island, a volcano in Antarctica. Deheyn DD, Gendreau P, Latz MI, Mar Environ Res 2005 Jul;60(1):1-33 3. Heart rate variability fraction-a new reportable measure of 24-hour R-R interval variation. Sosnowski M, Clark E, Macfarlane PW, Ann Noninvasive Electrocardiol 2005 Jan;10(1):7-15 4. High-power parametric amplification of 11.8-fs laser pulses with carrier-envelope phase control. Zinkstok RT, Witte S, Eikema KS, Opt Lett 2005 Jan 1;30(1):78-80 5. Passively mode-locked 914-nm Nd:YVO4 laser. Schlatter A, Krainer L, Paschotta R, Opt Lett 2005 Jan 1;30(1):44-6 6. Multidimensional, mapping-based complex wavelet transforms. Fernandes FC, van Spaendonck RL, IEEE Trans Image Process 2005 Jan;14(1):110-24.

Additional Info

Recognizes mouse CD90 Thy-1.1.