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Cdc34 (Ubc3, Ubiquitin Conjugating Enzyme)

Cdc34 (Ubc3, Ubiquitin Conjugating Enzyme)

Cat no: C2561-03C

Supplier: United States Biological
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Cdc34 is a ubiquitin conjugating enzyme (UBC) or E2 that has been shown to be essential for the G1 to S phase transition in S. cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In egg extracts of the frog Xenopus laevis, Cdc34 has been shown to be required for the onset of DNA replication and appears to function in a multi-protein complex. Also, Cdc34 is highly conserved among vertebrates and is likely to function in a similar manner in all eukaryotes to regulate the start of DNA synthesis. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C2561-03C
Reactivities: Human
Hosts: Mouse
Applications: Immunoprecipitation, Western Blot
Size: 100ug
Form: As reported
P type: Mab
Isotype: IgG1,k
Purity: Purified by Protein G affinity chromatography.
Alternative names: Cell division cycle 34, E2 CDC34, UBC3, UBE2R1, Ubiquitin carrier protein, Ubiquitin conjugating enzyme E232kD complementing, Ubiquitin protein ligase
References: 1.Tylers, M. and Willems, A.R. (1999) One ring to rule a superfamily of E3 ubiquitin ligases. Science 284:601-604. 2.Koepp, D.M., Harper, J.W. and Elledge, S.J. (1999) How cyclin became a cyclin: regulated proteolysis in the cell cycle. Cell 97:431-434. 3.Goebel, M.G., Yochem, J., Jentsch, S., McGrath, J.P., Varshavsky, A., and Byers, B. (1998) The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme. Science 241:1331-1335. 4.Yew, P.R. and Kirscher, M.W. (1997) Proteolysis and DNA replication: the CDC34 requirement in the Xenopus egg cell cycle. Science 277:1672-1676.
Additional info: Specific for human Cdc34.

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