CDw124, Recombinant, Human (Interleukin 4 Receptor alpha, Soluble, IL-4Ra)

Human IL-4 exerts its biological effects via signaling through its receptor system, IL-4R. There are two types of the IL-4 receptor complexes, the type I receptor consisting of the IL-4R alpha and the common gamma chain, yc and the type II receptor consisting of IL-4R alpha and the IL-13R alpha. The IL-4R alpha chain contains an extracellular domain of 207 amino acids, a transmembrane domain of 24 amino acids, and an intracellular domain of 569 amino acids. The secreted extracellular domain, sIL-4R alpha, also called CD124 is capable of blocking IL-4 activities. This receptor plays a major role in the regulation of the differentiation of naive CD4+ T cells and class switching to IgG1 and IgE. \n\nRecombinant human sIL-4Ra is a 209 amino acid protein which corresponds to the entire extracellular domain of IL-4Ra.\n\nAAпїЅSequence: GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH\n\nBiologicalпїЅActivity: \nThe ED50 as determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells is < 5ng/ml (in the presence of 0.5ng/ml IL-4), corresponding to a speci?c activity of (same/more than) 2x10e5 units/mg.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2587-60A

Size

3ug

Form

Supplied as a lyophilized powder from PBS, pH 7.0. Reconstitution in ddH2O to 0.1-1mg/ml. This solution can then be diluted into other aqueous buffers.

Purity

(same/more than) 97% by SDS-PAGE and HPLC analyses. Endotoxin: (same/less than)0.1ng/ug (1EU/ug)\n