The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the ovotransferrin present in the sample reacts with the anti-Ovotransferrin antibody which has been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound sample proteins by washing, anti-Ovotransferrin antibody conjugated with horseradish peroxidase (HRP) is added. This HRP-conjugated antibody forms a complex with the previously bound Ovotransferrin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of achromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme is proportional to the concentration of Ovotransferrin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentrationof Ovotransferrin in the test sample. The quantity of Ovotransferrin in the test sample can be interpolated from the calibration curve constructed from the calibrators, and corrected for sample dilution.
Quick Search
Products 
