Chitinase 3-like 1/YKL-40 BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse Chitinase 3-like 1 /YKL-40 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nChitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kD in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. \n\nAssay Range:\n62.5pg/ml-4000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nQ61362\n\nEntrez Gene ID:\n12654\n\nKit Components:\n96-well plate pre-coated with anti-Chitinase 3-like 1/YKL-40, Mouse\nLyophilized recombinant Chitinase 3-like 1/YKL-40, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143775

Size

96Tests

References

1. Ober, C., Tan, Z., Sun, Y., Possick, J. D., Pan, L., Nicolae, R., Radford, S., Parry, R. R., Heinzmann, A., Deichmann, K. A., Lester, L. A., Gern, J. E., Lemanske, R. F., Jr., Nicolae, D. L., Elias, J. A., Chupp, G. L. Effect of variation in CHI3L1 on serum YKL-40 level, risk of asthma, and lung function. New Eng. J. Med. 358: 1682-1691, 2008.\n2. Zhao, X., Tang, R., Gao, B., Shi, Y., Zhou, J., Guo, S., Zhang, J., Wang, Y., Tang, W., Meng, J., Li, S., Wang, H., and 10 others. Functional variants in the promoter region of Chitinase 3-like 1 (CHI3L1) and susceptibility to schizophrenia. Am. J. Hum. Genet. 80: 12-18, 2007. \n