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Chk2, active, Recombinant, Human (Checkpoint Kinase 2, Rad 53)

Cat no: C4201-15B

Chk2, active, Recombinant, Human (Checkpoint Kinase 2, Rad 53)

Chk2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner (1). Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogated both phosphorylation of p53 on Serine 20 and p53 stabilization (2). \n\nSource:\nRecombinant full-length human Chk2 was expressed by baculovirus in Sf 9 insect cells using an N-terminal GST tag. The gene accession number is NM_007194. \n\nMolecular Mass:\nThe approximate molecular weight is 88kD. \n\nActivity:\nThe specific activity of Chk2 was determined to be 729 nmol/min/mg using a synthetic peptide substrate (KKKVSRSGLYRSPSMPENLNRPR). \n\nStorage:\nThis product is stable at -70 degrees C for at least 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

C4201-15B

Size

10ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Purity

(same/more than) 90% by densitometry

References

1. Matsuoka, S. et al. (1998) Science 282:1893. \n2. Chehab, N.H. et al. (2000) Genes Dev. 14:278.

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