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Products Chloride Colorimetric Assay Kit
| Catalogue number: | 700610 |
| Weight: | 0 |
| Form: | 192 Well |
| P type: | Assay Kits |
| Shipping temp: | 4 |
| Storage temp: | 4 |
| Additional info: | Chloride is an essential mineral for humans. It is the major extracellular anion and is principally responsible for maintaining proper hydration, osmotic pressure, and normal cation-anion balance in the vascular and intestinal fluid compartments. Cayman's Colorimetric Chloride Assay provides a simple, reproducible, and sensitive tool for measuring chloride concentration in plasma, serum, urine, saliva, and cell culture lysates. The Chloride Assay is based upon the competition of Hg2+ and Fe2+ for thiocyanate. The preferred Hg-thiocyanate adduct exhibits no color. In the presence of chloride, Hg2+ forms mercuric chloride freeing up thiocyanate, which then binds to the available Fe2+ exhibiting an absorbance at 480 nm. The intensity of the color is directly proportional to the chloride concentration in the sample. |
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