Chloride Colorimetric Assay Kit

Chloride is the major extracellular anion and is principally responsible for maintaining proper hydration, osmotic pressure, and normal cation-anion balance in the vascular and intestinal fluid compartments. Cayman's Chloride Colorimetric Assay provides a simple, reproducible, and sensitive tool for measuring chloride concentration in plasma, serum, urine, saliva, and cell culture lysates. This assay is based upon the competition of Hg2+ and Fe2+ for thiocyanate. The preferred Hg-thiocyanate adduct exhibits no color. In the presence of chloride, Hg2+ forms mercuric chloride freeing up thiocyanate, which then binds to the available Fe2+ exhibiting an absorbance at 480 nm. The intensity of the color is directly proportional to the chloride concentration in the sample.

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SPECIFICATIONS

Catalog Number

700610

Form

192 Well

P Type

Assay Kits

Weight

0

Storage Temp

4

Shipping Temp

4

Additional Info

Chloride is an essential mineral for humans. It is the major extracellular anion and is principally responsible for maintaining proper hydration, osmotic pressure, and normal cation-anion balance in the vascular and intestinal fluid compartments. Cayman's Colorimetric Chloride Assay provides a simple, reproducible, and sensitive tool for measuring chloride concentration in plasma, serum, urine, saliva, and cell culture lysates. The Chloride Assay is based upon the competition of Hg2+ and Fe2+ for thiocyanate. The preferred Hg-thiocyanate adduct exhibits no color. In the presence of chloride, Hg2+ forms mercuric chloride freeing up thiocyanate, which then binds to the available Fe2+ exhibiting an absorbance at 480 nm. The intensity of the color is directly proportional to the chloride concentration in the sample.