Chorionic Gonadotropin beta, Total, Human (beta hCG) BioAssay(TM) ELISA Kit

The United States Biological B-hCG EIA test kit is mainly intended to quantitatively determine Total beta-hCG concentration in human serum. \n\nHuman chorionic gonadotropin (hCG) is a sialoglycoprotein with a molecular weight of approximately 46,000 daltons. HCG is initially secreted by the trophoblastic cells of the placenta shortly after implantation of the fertilized ovum into the uterine wall. The rapid rise in hCG serum levels after conception makes it an excellent marker for early confirmation and monitoring of pregnancy. Physiologically, hCG appears to maintain the corpus luteum, thereby allowing synthesis of progesterone and estrogens that support the endometrium. As uncomplicated pregnancies progress, the placenta assumes the production of these hormones. The serum hCG levels increase to a peak concentration, then decrease and plateau. HCG circulates as the intact molecule in the serum of normal women who have an uncomplicated pregnancy. The subunits are cleared rapidly and excreted by the kidney. The placental hormone, hCG, is similar to luteinizing hommone (LH), follicle simulating hormone (FSH), and human thyroid stimulating hormone (hTSH). All are glycoproteins consisting of two noncovalently bound dissimilar subunits, designated alpha and beta, with attached carbohydrate sidechains. The alpha subunits of these glycoproteins are very similar. In contrast, the beta subunit portions determine the biological and immunochemical specificities. The beta subunits of hCG and LH exhibit considerable homology in amino acid content. Amino acid residues specific for the beta subunit of hCG confer the immuno-chemical specificity. With the availability of sensitive quantitative assays for the measurement of serum b-hCG, it has been shown that hCG levels can be useful in predicting spontaneous abortions, aiding in the detection of ectopic pregnancy and multiple gestation. Elevated levels of hCG have also been detected in serum from patients with abnormal \nphysiological con ditions not related to pregnancy. The hCG EIA test provides a rapid, sensitive and reliable assay. The antibodies developed for the test will determine a minimal concentration of 2 mIU/ml.\n\nDetection Range: 0-300 mIU/mL \n\nSample: 10ul serum \n\nSpecificity: ~97% \n\nSensitivity: 2.0 mIU/ml \n\nTotal Time: ~80 min \n\n\nKit Components: \nEach kit contains the following components in sufficient quantities to perform the number of tests indicated. All reactive reagents contain 0.1% sodium azide as a preservative \n1. Microtiter Plate\n2. Conjugate\n3. Calibrator\n4. Positive Control\n5. Negative Control\n6. TMB\n7. Wash Buffer, 10X Diluted wash buffer to 1X\n8. Sample Diluent\n9. Stop Solution \n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5069-07

Size

96Tests

Applications

ELISA

References

1. Stenman U.H. et. al., Obstet. Gynecol. 1882; 59:375-377 2. Kosasa T.S. J. Reprod. Med. 1981; 26: 201 3. Dipietro D.L. Laboratory Management 1981; 19: 1 4. Uotilia M. et. al., J. Immunol. Methods 1981; 42: 11-15 5. Masseyeff R. and Maiolini R. J. Immunol Methods 1975; 8: 233